cdna libraries preparation Search Results


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LGC Genomics GmbH rna extractions, cdna synthesis library preparation and sequencing
Rna Extractions, Cdna Synthesis Library Preparation And Sequencing, supplied by LGC Genomics GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rna extractions, cdna synthesis library preparation and sequencing - by Bioz Stars, 2026-07
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Vertis Biotechnologie cdna library preparation
S . Typhimurium strain 4/74 was grown within macrophages for 8 hours using the gentamicin protection assay, and bacterial RNA was isolated using TRIzol (Materials and Methods). The <t>cDNA</t> generated from total RNA was sequenced either directly for gene/sRNA expression analysis (RNA-seq) or after enrichment of primary transcripts (dRNA-seq), and compared with data from 4/74 grown to ESP . The graphs show representations of sequence reads mapped uniquely against the 4/74 genome in different conditions. Transcript per Million (TPM) analysis was used to calculate gene expression values from the number of sequence reads mapped against the 4/74 genome. The promoter usage value (PUV) indicates the TPM value of the first 10 nucleotides from the transcription start sites (TSS) in the direction of transcription, and represents promoter strength. Each curved arrow indicates location of TSS upstream of the respective gene; the width and height of each curved arrow is proportional to TSS expression, based on relative PUV, macrophage versus ESP.
Cdna Library Preparation, supplied by Vertis Biotechnologie, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
cdna library preparation - by Bioz Stars, 2026-07
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Genotypic Technology Pvt Ltd cdna library preparation
S . Typhimurium strain 4/74 was grown within macrophages for 8 hours using the gentamicin protection assay, and bacterial RNA was isolated using TRIzol (Materials and Methods). The <t>cDNA</t> generated from total RNA was sequenced either directly for gene/sRNA expression analysis (RNA-seq) or after enrichment of primary transcripts (dRNA-seq), and compared with data from 4/74 grown to ESP . The graphs show representations of sequence reads mapped uniquely against the 4/74 genome in different conditions. Transcript per Million (TPM) analysis was used to calculate gene expression values from the number of sequence reads mapped against the 4/74 genome. The promoter usage value (PUV) indicates the TPM value of the first 10 nucleotides from the transcription start sites (TSS) in the direction of transcription, and represents promoter strength. Each curved arrow indicates location of TSS upstream of the respective gene; the width and height of each curved arrow is proportional to TSS expression, based on relative PUV, macrophage versus ESP.
Cdna Library Preparation, supplied by Genotypic Technology Pvt Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cdna+libraries+preparation/pmc05593950-288-1-16?v=Genotypic+Technology+Pvt+Ltd
Average 90 stars, based on 1 article reviews
cdna library preparation - by Bioz Stars, 2026-07
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WuXi AppTec cdna libraries preparation and sequencing
S . Typhimurium strain 4/74 was grown within macrophages for 8 hours using the gentamicin protection assay, and bacterial RNA was isolated using TRIzol (Materials and Methods). The <t>cDNA</t> generated from total RNA was sequenced either directly for gene/sRNA expression analysis (RNA-seq) or after enrichment of primary transcripts (dRNA-seq), and compared with data from 4/74 grown to ESP . The graphs show representations of sequence reads mapped uniquely against the 4/74 genome in different conditions. Transcript per Million (TPM) analysis was used to calculate gene expression values from the number of sequence reads mapped against the 4/74 genome. The promoter usage value (PUV) indicates the TPM value of the first 10 nucleotides from the transcription start sites (TSS) in the direction of transcription, and represents promoter strength. Each curved arrow indicates location of TSS upstream of the respective gene; the width and height of each curved arrow is proportional to TSS expression, based on relative PUV, macrophage versus ESP.
Cdna Libraries Preparation And Sequencing, supplied by WuXi AppTec, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cdna+libraries+preparation/pmc06700472__mmc1-125-5-9?v=WuXi+AppTec
Average 90 stars, based on 1 article reviews
cdna libraries preparation and sequencing - by Bioz Stars, 2026-07
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Vertis Biotechnologie primary and random cdna library constructions and next-generation sequencing
S . Typhimurium strain 4/74 was grown within macrophages for 8 hours using the gentamicin protection assay, and bacterial RNA was isolated using TRIzol (Materials and Methods). The <t>cDNA</t> generated from total RNA was sequenced either directly for gene/sRNA expression analysis (RNA-seq) or after enrichment of primary transcripts (dRNA-seq), and compared with data from 4/74 grown to ESP . The graphs show representations of sequence reads mapped uniquely against the 4/74 genome in different conditions. Transcript per Million (TPM) analysis was used to calculate gene expression values from the number of sequence reads mapped against the 4/74 genome. The promoter usage value (PUV) indicates the TPM value of the first 10 nucleotides from the transcription start sites (TSS) in the direction of transcription, and represents promoter strength. Each curved arrow indicates location of TSS upstream of the respective gene; the width and height of each curved arrow is proportional to TSS expression, based on relative PUV, macrophage versus ESP.
Primary And Random Cdna Library Constructions And Next Generation Sequencing, supplied by Vertis Biotechnologie, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cdna+libraries+preparation/pmc06091082-265-85-72?v=Vertis+Biotechnologie
Average 90 stars, based on 1 article reviews
primary and random cdna library constructions and next-generation sequencing - by Bioz Stars, 2026-07
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CapitalBio Corporation quick cdna library preparation kit
S . Typhimurium strain 4/74 was grown within macrophages for 8 hours using the gentamicin protection assay, and bacterial RNA was isolated using TRIzol (Materials and Methods). The <t>cDNA</t> generated from total RNA was sequenced either directly for gene/sRNA expression analysis (RNA-seq) or after enrichment of primary transcripts (dRNA-seq), and compared with data from 4/74 grown to ESP . The graphs show representations of sequence reads mapped uniquely against the 4/74 genome in different conditions. Transcript per Million (TPM) analysis was used to calculate gene expression values from the number of sequence reads mapped against the 4/74 genome. The promoter usage value (PUV) indicates the TPM value of the first 10 nucleotides from the transcription start sites (TSS) in the direction of transcription, and represents promoter strength. Each curved arrow indicates location of TSS upstream of the respective gene; the width and height of each curved arrow is proportional to TSS expression, based on relative PUV, macrophage versus ESP.
Quick Cdna Library Preparation Kit, supplied by CapitalBio Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cdna+libraries+preparation/pmc11703948-140-7-12?v=CapitalBio+Corporation
Average 90 stars, based on 1 article reviews
quick cdna library preparation kit - by Bioz Stars, 2026-07
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Q2 Lab Solutions cdna library preparation
S . Typhimurium strain 4/74 was grown within macrophages for 8 hours using the gentamicin protection assay, and bacterial RNA was isolated using TRIzol (Materials and Methods). The <t>cDNA</t> generated from total RNA was sequenced either directly for gene/sRNA expression analysis (RNA-seq) or after enrichment of primary transcripts (dRNA-seq), and compared with data from 4/74 grown to ESP . The graphs show representations of sequence reads mapped uniquely against the 4/74 genome in different conditions. Transcript per Million (TPM) analysis was used to calculate gene expression values from the number of sequence reads mapped against the 4/74 genome. The promoter usage value (PUV) indicates the TPM value of the first 10 nucleotides from the transcription start sites (TSS) in the direction of transcription, and represents promoter strength. Each curved arrow indicates location of TSS upstream of the respective gene; the width and height of each curved arrow is proportional to TSS expression, based on relative PUV, macrophage versus ESP.
Cdna Library Preparation, supplied by Q2 Lab Solutions, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
cdna library preparation - by Bioz Stars, 2026-07
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BGI Tech Solutions Co Ltd purification of mrna, synthesis of cdna library and sequencing
S . Typhimurium strain 4/74 was grown within macrophages for 8 hours using the gentamicin protection assay, and bacterial RNA was isolated using TRIzol (Materials and Methods). The <t>cDNA</t> generated from total RNA was sequenced either directly for gene/sRNA expression analysis (RNA-seq) or after enrichment of primary transcripts (dRNA-seq), and compared with data from 4/74 grown to ESP . The graphs show representations of sequence reads mapped uniquely against the 4/74 genome in different conditions. Transcript per Million (TPM) analysis was used to calculate gene expression values from the number of sequence reads mapped against the 4/74 genome. The promoter usage value (PUV) indicates the TPM value of the first 10 nucleotides from the transcription start sites (TSS) in the direction of transcription, and represents promoter strength. Each curved arrow indicates location of TSS upstream of the respective gene; the width and height of each curved arrow is proportional to TSS expression, based on relative PUV, macrophage versus ESP.
Purification Of Mrna, Synthesis Of Cdna Library And Sequencing, supplied by BGI Tech Solutions Co Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
purification of mrna, synthesis of cdna library and sequencing - by Bioz Stars, 2026-07
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Nextera AS cdna libraries nextera xt kit
S . Typhimurium strain 4/74 was grown within macrophages for 8 hours using the gentamicin protection assay, and bacterial RNA was isolated using TRIzol (Materials and Methods). The <t>cDNA</t> generated from total RNA was sequenced either directly for gene/sRNA expression analysis (RNA-seq) or after enrichment of primary transcripts (dRNA-seq), and compared with data from 4/74 grown to ESP . The graphs show representations of sequence reads mapped uniquely against the 4/74 genome in different conditions. Transcript per Million (TPM) analysis was used to calculate gene expression values from the number of sequence reads mapped against the 4/74 genome. The promoter usage value (PUV) indicates the TPM value of the first 10 nucleotides from the transcription start sites (TSS) in the direction of transcription, and represents promoter strength. Each curved arrow indicates location of TSS upstream of the respective gene; the width and height of each curved arrow is proportional to TSS expression, based on relative PUV, macrophage versus ESP.
Cdna Libraries Nextera Xt Kit, supplied by Nextera AS, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cdna libraries nextera xt kit - by Bioz Stars, 2026-07
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MetWare Ltd rna quantification and qualification, cdna libraries preparation, clustering and sequencing and data analyses
S . Typhimurium strain 4/74 was grown within macrophages for 8 hours using the gentamicin protection assay, and bacterial RNA was isolated using TRIzol (Materials and Methods). The <t>cDNA</t> generated from total RNA was sequenced either directly for gene/sRNA expression analysis (RNA-seq) or after enrichment of primary transcripts (dRNA-seq), and compared with data from 4/74 grown to ESP . The graphs show representations of sequence reads mapped uniquely against the 4/74 genome in different conditions. Transcript per Million (TPM) analysis was used to calculate gene expression values from the number of sequence reads mapped against the 4/74 genome. The promoter usage value (PUV) indicates the TPM value of the first 10 nucleotides from the transcription start sites (TSS) in the direction of transcription, and represents promoter strength. Each curved arrow indicates location of TSS upstream of the respective gene; the width and height of each curved arrow is proportional to TSS expression, based on relative PUV, macrophage versus ESP.
Rna Quantification And Qualification, Cdna Libraries Preparation, Clustering And Sequencing And Data Analyses, supplied by MetWare Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
rna quantification and qualification, cdna libraries preparation, clustering and sequencing and data analyses - by Bioz Stars, 2026-07
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Basler cdna library prepared from lps-stimulated raw264.7 cells
S . Typhimurium strain 4/74 was grown within macrophages for 8 hours using the gentamicin protection assay, and bacterial RNA was isolated using TRIzol (Materials and Methods). The <t>cDNA</t> generated from total RNA was sequenced either directly for gene/sRNA expression analysis (RNA-seq) or after enrichment of primary transcripts (dRNA-seq), and compared with data from 4/74 grown to ESP . The graphs show representations of sequence reads mapped uniquely against the 4/74 genome in different conditions. Transcript per Million (TPM) analysis was used to calculate gene expression values from the number of sequence reads mapped against the 4/74 genome. The promoter usage value (PUV) indicates the TPM value of the first 10 nucleotides from the transcription start sites (TSS) in the direction of transcription, and represents promoter strength. Each curved arrow indicates location of TSS upstream of the respective gene; the width and height of each curved arrow is proportional to TSS expression, based on relative PUV, macrophage versus ESP.
Cdna Library Prepared From Lps Stimulated Raw264.7 Cells, supplied by Basler, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cdna+libraries+preparation/pm16415166-207-12-0?v=Basler
Average 90 stars, based on 1 article reviews
cdna library prepared from lps-stimulated raw264.7 cells - by Bioz Stars, 2026-07
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Oxford Nanopore cdna synthesis sequencing library preparation
S . Typhimurium strain 4/74 was grown within macrophages for 8 hours using the gentamicin protection assay, and bacterial RNA was isolated using TRIzol (Materials and Methods). The <t>cDNA</t> generated from total RNA was sequenced either directly for gene/sRNA expression analysis (RNA-seq) or after enrichment of primary transcripts (dRNA-seq), and compared with data from 4/74 grown to ESP . The graphs show representations of sequence reads mapped uniquely against the 4/74 genome in different conditions. Transcript per Million (TPM) analysis was used to calculate gene expression values from the number of sequence reads mapped against the 4/74 genome. The promoter usage value (PUV) indicates the TPM value of the first 10 nucleotides from the transcription start sites (TSS) in the direction of transcription, and represents promoter strength. Each curved arrow indicates location of TSS upstream of the respective gene; the width and height of each curved arrow is proportional to TSS expression, based on relative PUV, macrophage versus ESP.
Cdna Synthesis Sequencing Library Preparation, supplied by Oxford Nanopore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cdna synthesis sequencing library preparation - by Bioz Stars, 2026-07
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Image Search Results


S . Typhimurium strain 4/74 was grown within macrophages for 8 hours using the gentamicin protection assay, and bacterial RNA was isolated using TRIzol (Materials and Methods). The cDNA generated from total RNA was sequenced either directly for gene/sRNA expression analysis (RNA-seq) or after enrichment of primary transcripts (dRNA-seq), and compared with data from 4/74 grown to ESP . The graphs show representations of sequence reads mapped uniquely against the 4/74 genome in different conditions. Transcript per Million (TPM) analysis was used to calculate gene expression values from the number of sequence reads mapped against the 4/74 genome. The promoter usage value (PUV) indicates the TPM value of the first 10 nucleotides from the transcription start sites (TSS) in the direction of transcription, and represents promoter strength. Each curved arrow indicates location of TSS upstream of the respective gene; the width and height of each curved arrow is proportional to TSS expression, based on relative PUV, macrophage versus ESP.

Journal: PLoS Pathogens

Article Title: RNA-seq Brings New Insights to the Intra-Macrophage Transcriptome of Salmonella Typhimurium

doi: 10.1371/journal.ppat.1005262

Figure Lengend Snippet: S . Typhimurium strain 4/74 was grown within macrophages for 8 hours using the gentamicin protection assay, and bacterial RNA was isolated using TRIzol (Materials and Methods). The cDNA generated from total RNA was sequenced either directly for gene/sRNA expression analysis (RNA-seq) or after enrichment of primary transcripts (dRNA-seq), and compared with data from 4/74 grown to ESP . The graphs show representations of sequence reads mapped uniquely against the 4/74 genome in different conditions. Transcript per Million (TPM) analysis was used to calculate gene expression values from the number of sequence reads mapped against the 4/74 genome. The promoter usage value (PUV) indicates the TPM value of the first 10 nucleotides from the transcription start sites (TSS) in the direction of transcription, and represents promoter strength. Each curved arrow indicates location of TSS upstream of the respective gene; the width and height of each curved arrow is proportional to TSS expression, based on relative PUV, macrophage versus ESP.

Article Snippet: The cDNA library preparation and Illumina sequencing was done by Vertis Biotechnologie AG (Freising, Germany).

Techniques: Isolation, Generated, Expressing, RNA Sequencing, Sequencing, Gene Expression